Topilo Topnost CBD
(mg/mL)
Temperatura določanja
Vir
Etanol 35 / (57)
23,59 / (59)
36 / (58)
Metanol 30 / (57)
DMSO 60 / (57)
23,59 / (59)
36 / (58)
DMF 50 / (57)
36 / (58)
Tekoči parafin 19,65 Sobna temperatura (60)
Deviško olivno olje > 300 Sobna temperatura (60)
80% propilenglikol–20% voda (w/w) 16,23 Sobna temperatura (60) 82% PEG 400–18% voda (w/w) 45,16 Sobna temperatura (60)
Propilenglikol : voda = 7 : 3 1,80 32 °C (61)
Propilenglikol : voda : etanol = 4 : 5 : 4 5,09 32 °C (61)
Vsi podatki o topnosti CBD v etanolu, metanolu, DMSO in DMF so pridobljeni iz varnostnih listov. Glede na objavljene podatke, naj bi bila topnost CBD v etanolu 31,5 mg/mL, kar se zelo razlikuje od našega rezultata, ki je 3745,86 mg/g etanola. Podobno so precejšnje razlike prisotne tudi pri ostalih treh topilih (57, 58, 59).
42
Ostali podatki o topnosti so izpisani iz znanstvenih člankov, kjer je opisano, da so topnost določali s shake-flask metodo. Topnost CBD v olivnem olju naj bi bila več kot 300 mg/mL, kar se ujema z našim rezultatom, ki je 379,74 mg/mL. Topnost CBD v 80% propilenglikolu in 20%
vodi naj bi bila 16,23 mg/mL. Mi smo v 85% propilenglikolu in 15% vodi (w/w) dobili topnost 20,37 mg/g topila (okoli 20 mg/mL topila). Tudi ta rezultat je smiseln, saj se z večanjem deleža vode v propilenglikolu znižuje topnost CBD.
Casiraghi in sodelavci so topnost CBD določili tudi v raztopini z 82 % PEG 400 in 18 % vode (60). Sklepamo, da pri tem razmerju PEG 400 in vode še ni prišlo do razslojevanja, kot je v našem primeru, ko smo topnost CBD določali v raztopini z 80 % PEG 400 in 20 % vode.
43
5 SKLEP
S pregledom spletnega trga kapljic in pršil s CBD smo ugotovili, da se kot nosilna topila najpogosteje uporabljajo MCT olje, olje konopljinih semen, olivno olje in njihove zmesi. Na slovenskem trgu je bilo 61,2 % izdelkov v olju konopljinih semen, 14,3 % v MCT olju in 8,2
% v olivnem olju. Na tujem trgu pa je bilo 62,4 % izdelkov v MCT olju, 14,9 % izdelkov v olju konopljinih semen in 3,5 % izdelkov v olivnem olju. Zmesi olj so skupno predstavljale 9,5 % vseh nosilnih topil. Največkrat prisotna zmes je bila zmes MCT olja z oljem konopljinih semen.
Med rastlinskimi olji je bila topnost CBD pri 25 °C najvišja v MCT olju (681,31 mg/mL topila), sledili so čijevo olje (410,40 mg/mL topila), olje konopljinih semen (400,08 mg/mL topila) in olivno olje (379,74 mg/mL topila). Čijevo olje, olje konopljinih semen in olivno olje spadajo med LCT olja, poleg tega vsi večinoma vsebujejo trigliceride z nenasičenimi maščobnimi kislinami. To je razlog za podobno topnost pri teh topilih.
Topnost v propilenglikolu in njegovih vodnih raztopinah smo določili pri 26 °C. Topnost CBD v propilenglikolu je bila 780,56 mg/g topila. V propilenglikolu s 5% (w/w) vsebnostjo vode je bila topnost CBD le še 269,89 mg/g topila. V raztopini z 10 % (w/w) vode in 90 % (w/w) propilenglikola je bila topnost CBD 85,68 mg/g topila. S povečanjem masnega deleža vode se je topnost poslabšala.
V 95% (w/w) glicerolu in 5% (w/w) vodi je bila topnost CBD tako nizka, da kvantifikacija s HPLC metodo ni bila možna. V triacetinu je bila topnost CBD 1333,61 mg/g topila. V PEG 400 je bila topnost višja od 1316,53 mg CBD/g topila. V trietil citratu je bila topnost CBD višja od 1172,69 mg/g topila. V tej skupini topil smo topnost določali pri 25–26 °C.
Za razliko od nizke topnosti v vodi je bil CBD zelo dobro topen v večini ostalih topil. Še posebej v laboratorijskih topilih (pri 25,5–27 °C). V acetonu je bila njegova topnost kar 5490,01 mg/g topila. Sledila sta metanol (4711,02 mg/g topila) in dietileter (4681,82 mg/g topila). Izmed izbranih laboratorijskih topil je bila topnost najnižja v izooktanu, v katerem je bila 342,30 mg/g topila.
Ob določanju topnosti CBD v zmeseh glicerol–96 % etanol je prišlo ob dodatku CBD do ločitve etanolne faze od glicerolne. Podobno je do ločitve faz prišlo tudi ob dodatku CBD v raztopine PEG 400 in vode, ki so vsebovale vsaj 20% masni delež vode. Razlog za to bi lahko bila lipofilnost CBD.
44
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49
7 PRILOGA
Izdelki na spletu, ob iskanju s ključnima besedama »CBD kapljice« ali »CBD pršilo«, (datum iskanja: 28. 6. 2021).
Izdelek Volum en (mL)
Topilo Vsebnost
CBD (w/V %)
proizvajalec Cena (€)
3 Hemptouch 22 https://www.zeleni-
1,5 Hemptouch 23 https://www.zeleni-
5 Dr. Gosar 18 https://konopljazdravi.si/
cbd-konopljine-kapljice/
CBD kapljice
10 Olje iz
konopljinih semen
12 Be Hempy 73 https://www.sanolabor.si
50
Canadoc 115 https://www.slovenska- konoplja.si/izdelek/cbd-kapljice-1500mg/
CBD kapljice
15 Olivno olje 5 EkoKor 27 https://semenakonoplje.si
10 Hempika 64,90
https://cbd-
1 Hempika 20,90 https://www.eden-
10 BioBloom 59,90 https://mojcbd.si/izdelek/
3 Agrosloven 19,99 https://www.agrosloven.
10 MCT olje 20 Smart 53,91 https://www.cbdshop.si/s
mart-20-cbd-kapljice
5 Bioteca 20,79 https://bioteca.eu/trgovin a/cbd-kapljice-5-10ml/
CBD kapljice
10 Olje iz
konopljinih semen
10 Eko-sol 38,99 https://www.dm.si/eko- sol-cbd-kapljice-10-
1000-mg-p3830060541185.html
51
12 Dolocan 38 https://greenpassion.si/sp
5 Dr. Natura 34,90 https://www.drnatura.si/c
5 Zmüesli 35 https://www.zmuesli.si/n aravni-izdelki/kapljice
HempLight 44,90 https://www.hemplight.n
10 Suprahemp 66,05 https://www.cbd-izdelki.si/izdelek/suprahe
5 Konopko 27 https://www.konopko.si/i
52
10 MCT olje 10 Cannatim 46,90 https://cannatim.si/izdele k/cbd-kapljice-1000mg/
CBD pršilo
30 MCT olje 1 Cannatim 19,90 https://cannatim.si/izdele k/cbd-prsilo-300mg/
5 KONOBI 39,90 https://vrsicek.si/product/
5 IIK 29,70 https://www.mkomel.co
5 Herbio 29 https://herbio.org/izdelek /cbd-konopljine-kapljice-5/
53
5 VitalAbo 28,99 https://www.vitalabo.si/c ksilitolom in olja iz konopljinih semen
0,984 BetterYou 33,90
https://www.e-
54
Izdelki na internetu, ob iskanju v angleškem jeziku s ključnima besedama »CBD drops« ali
»CBD spray« (datum iskanja: 28. 6. 2021)
Izdelek Volumen (mL)
Topilo Vsebnost CBD (w/V %)
Proizvajalec Cena spletna stran
CBD
10 Olivno olje 5 cibdol 29,95€ https://www.cibdol.com/
cbd-oil/cbd-oil-5
55
56
CBDWorld 20€ https://www.cbdworld.it/
farms-cbd-tincture-full-57
Joy organics 69,95 USD
58
76,30 € https://advanced-botanics.com/product/cb
59
60
15 H Drop 79€ https://www.hdrop.com/
Hemplucid 84,95 USD
61
Harmony 18,96€ https://www.canatura.co
10 VAAY 54,90€ https://vaay.com/en/prod
ss.co/cbd-products/cbd-62
10 MCT olje 10 healthspan / https://www.amazon.co.
63
64
3 PHYTO+ 22,95€ https://www.phytopluscb
shop/food-65
drops/valencia-orange-66
1,33 Seven Peak 25€ https://sevenpeakcbd.co
67
cbd.com/product/cbd-68
69 CBD
kapljice
15 Kokosovo
olje
3,2 Care by design
/ https://www.cbd.org/pro duct/401-drops-15ml CBD
kapljice
10 MCT olje 5 Phytocan 21,99
funtov
https://thecbdsupplier.co.
uk/product/phytocan- gold-cbd-oil-natural-flavour/
CBD kapljice
10 Olje
konopljinih semen
10 aire 70
funtov
https://www.roots-wellness.co.uk/products/
aire-cbd-010-cbd-oil CBD
kapljice
10 Olje
konopljinih semen
15 ENDOCA / https://www.endoca.com
/en-gb/product/raw- hemp-oil-drops-1500mg#
70